Soha M. Abd El Dayem
National Research Centre, Egypt
Title: Relationship of nitric oxide genotype to diabetic nephropathy and atherosclerosis of type 1 diabetic patients
Biography
Biography: Soha M. Abd El Dayem
Abstract
Objective: Assessment of nitric oxide genotype in diabetic patients and to evaluate its relationship to diabetic nephropathy and atherosclerosis of type 1 diabetic patients.
Patients and methods: The study included 65 type 1 diabetic patients and 30 age and sex matched healthy volunteers. The mean age of patients was 17.99 ± 2.59, mean duration of diabetes was 10.91 ± 3.54, mean onset of disease was 7.00 ± 3.28. Blood samples were taken for assessment of glycosylated haemoglobin (HbA1), lipid profile, oxidized low-density lipoprotein (OxLDL) and serum level of nitric oxide by enzyme linked immunosorbent assay (ELISA) technique. Also, nitric oxide genotype was done. Urine samples were taken for assessment of albumin/creatinine ratio. Carotid intima-media thickness (cIMT) and renal dopplar via ultrasound were also done.
Results: Nitric oxide was significantly lower, while lipid profile, OxLDL and albumin/creatinine ratio, cIMT and resistivity index were significantly higher in diabetic patients. No significant difference of nitric oxide genotype was found in diabetics and control. Nitric oxide was significantly lower, while OxLDL, albumin/ creatinine ratio and lipid profile were significantly higher in nitric oxide homozygous genotype.
Conclusion: Diabetic patients had a low level of nitric oxide and early atherosclerosis. Nitric oxide homozygous genotype is associated with diabetic nephropathy and atherosclerosis.
